575 people died it sounds like a death toll from the earthquake the Siddha
toll from the earthquake the Siddha
toll from the earthquake the Siddha means a natural disaster normally saw
means a natural disaster normally saw
means a natural disaster normally saw coming
coming
coming and fortunately afterward as a health
and fortunately afterward as a health
and fortunately afterward as a health clinic volunteers few years ago it was
clinic volunteers few years ago it was
clinic volunteers few years ago it was just another tuberculosis deaths at the
just another tuberculosis deaths at the
just another tuberculosis deaths at the time in reporter ladies and gentlemen
time in reporter ladies and gentlemen
time in reporter ladies and gentlemen today I will be telling you a story a
today I will be telling you a story a
today I will be telling you a story a story about how myself a high school
story about how myself a high school
story about how myself a high school students soccer tackle one of the
students soccer tackle one of the
students soccer tackle one of the world’s most notorious diseases
world’s most notorious diseases
world’s most notorious diseases tuberculosis now the journey all started
tuberculosis now the journey all started
tuberculosis now the journey all started back in the summer of my freshman year
back in the summer of my freshman year
back in the summer of my freshman year initially with my father – in a small
initially with my father – in a small
initially with my father – in a small village in sight don’t country the
village in sight don’t country the
village in sight don’t country the village is located in the mountainous
village is located in the mountainous
village is located in the mountainous areas and borders between Thailand and
areas and borders between Thailand and
areas and borders between Thailand and hikmah and it is in fact one of the most
hikmah and it is in fact one of the most
hikmah and it is in fact one of the most TV that endemic regions of the country
TV that endemic regions of the country
TV that endemic regions of the country now while I was volunteering at the
now while I was volunteering at the
now while I was volunteering at the village it’s not the difficulty the
village it’s not the difficulty the
village it’s not the difficulty the people in the village undergoing to
people in the village undergoing to
people in the village undergoing to diagnose four different diseases
diagnose four different diseases
diagnose four different diseases especially to the closest however what
especially to the closest however what
especially to the closest however what shocked me the most was the fact that a
shocked me the most was the fact that a
shocked me the most was the fact that a psycho
psycho
psycho district only has 62,000 people but it
district only has 62,000 people but it
district only has 62,000 people but it has more annual TB deaths in the entire
has more annual TB deaths in the entire
has more annual TB deaths in the entire United States so the major question that
United States so the major question that
United States so the major question that immediately came up in my mind is why is
immediately came up in my mind is why is
immediately came up in my mind is why is it that a disease that is already
it that a disease that is already
it that a disease that is already spherical a disease that is already
spherical a disease that is already
spherical a disease that is already preventable
preventable
preventable why is it still taking away countless
why is it still taking away countless
why is it still taking away countless lives and we do with this burning
lives and we do with this burning
lives and we do with this burning question in mind I came back on the body
question in mind I came back on the body
question in mind I came back on the body tension and I started to do more
tension and I started to do more
tension and I started to do more research and I realize that the problem
research and I realize that the problem
research and I realize that the problem actually isn’t about you the closest the
actually isn’t about you the closest the
actually isn’t about you the closest the problem actually is about to be however
problem actually is about to be however
problem actually is about to be however the problem is about the drug-resistant
according to the w-h-o only one out of
according to the w-h-o only one out of four TV patients get diagnosed for 12
four TV patients get diagnosed for 12
four TV patients get diagnosed for 12 resistant in other words 75% of TB
resistant in other words 75% of TB
resistant in other words 75% of TB patients are actually totally unaware
patients are actually totally unaware
patients are actually totally unaware whether they have a drug business dick
whether they have a drug business dick
whether they have a drug business dick forms of disease and therefore our way
forms of disease and therefore our way
forms of disease and therefore our way of wasting their money wasted their time
of wasting their money wasted their time
of wasting their money wasted their time and risking their lives on antibiotics
and risking their lives on antibiotics
and risking their lives on antibiotics and treatments that may not even be
and treatments that may not even be
and treatments that may not even be worthy and the root cause of all these
worthy and the root cause of all these
worthy and the root cause of all these problems because it takes about 30 days
problems because it takes about 30 days
problems because it takes about 30 days to detect drug-resistant TB now it is
to detect drug-resistant TB now it is
to detect drug-resistant TB now it is very important that we understand the
very important that we understand the
very important that we understand the severity of the consequences that
severity of the consequences that
severity of the consequences that follows because it is not really a
follows because it is not really a
follows because it is not really a problem of waiting for 30 days and
problem of waiting for 30 days and
problem of waiting for 30 days and therefore risking the patient’s lives
therefore risking the patient’s lives
therefore risking the patient’s lives and wasting their time and money but it
and wasting their time and money but it
and wasting their time and money but it is also a problem about the future the
is also a problem about the future the
is also a problem about the future the future where we ourselves are at fault
future where we ourselves are at fault
future where we ourselves are at fault for turning the disease that is Iranian
for turning the disease that is Iranian
for turning the disease that is Iranian steerable back into an incurable disease
steerable back into an incurable disease
steerable back into an incurable disease and so when that happens when
and so when that happens when
and so when that happens when drug-resistant TB takes control
drug-resistant TB takes control
drug-resistant TB takes control when had last effects anti biotics along
when had last effects anti biotics along
when had last effects anti biotics along forwards but then what we do so with
forwards but then what we do so with
forwards but then what we do so with this project issue in mind I realized
this project issue in mind I realized
this project issue in mind I realized that the key to solving all of these
that the key to solving all of these
that the key to solving all of these cascading problems is to shorten the
cascading problems is to shorten the
cascading problems is to shorten the diagnostic process for drug resistant to
diagnostic process for drug resistant to
diagnostic process for drug resistant to use now to do that is first necessary
use now to do that is first necessary
use now to do that is first necessary that we understand how the convention
that we understand how the convention
that we understand how the convention it works so for example the connective
it works so for example the connective
it works so for example the connective most widely used method involves
most widely used method involves
most widely used method involves treating at the examples and antibiotics
treating at the examples and antibiotics
treating at the examples and antibiotics and then observing whether the TTB
and then observing whether the TTB
and then observing whether the TTB samples actually grow and reproduce so
samples actually grow and reproduce so
samples actually grow and reproduce so for example if the TV samples grow into
for example if the TV samples grow into
for example if the TV samples grow into a visible colony that it is considered
a visible colony that it is considered
a visible colony that it is considered drug resistant now this method works for
drug resistant now this method works for
drug resistant now this method works for all types of bacteria usually you’ll
all types of bacteria usually you’ll
all types of bacteria usually you’ll need quite a few hours however since TV
need quite a few hours however since TV
need quite a few hours however since TV bacteria is one of the world’s slowest
bacteria is one of the world’s slowest
bacteria is one of the world’s slowest growth bacteria detecting drug-resistant
growth bacteria detecting drug-resistant
growth bacteria detecting drug-resistant TB takes about 30 days
fortunately one thing I recall from my
fortunately one thing I recall from my internship at red cross a concept known
internship at red cross a concept known
internship at red cross a concept known as reputation which is essentially blood
as reputation which is essentially blood
as reputation which is essentially blood fontaine now I know that the same thing
fontaine now I know that the same thing
fontaine now I know that the same thing was happening with TV bacteria which has
was happening with TV bacteria which has
was happening with TV bacteria which has a natural tendency to talk together into
a natural tendency to talk together into
a natural tendency to talk together into a large boost causing the proteins to be
a large boost causing the proteins to be
a large boost causing the proteins to be trapped with anything and so I realized
trapped with anything and so I realized
trapped with anything and so I realized that if I could somehow transform the TV
that if I could somehow transform the TV
that if I could somehow transform the TV samples into a single cell suspension
samples into a single cell suspension
samples into a single cell suspension and then create a sensor to measure the
and then create a sensor to measure the
and then create a sensor to measure the change in protein secretion this
change in protein secretion this
change in protein secretion this measurement would possibly indicate the
measurement would possibly indicate the
measurement would possibly indicate the bacterias metabolic activity eliminating
bacterias metabolic activity eliminating
bacterias metabolic activity eliminating the requirement from similar growth now
the requirement from similar growth now
the requirement from similar growth now let’s take a look at this idea a bit
let’s take a look at this idea a bit
let’s take a look at this idea a bit more closely so first we treat the TV
more closely so first we treat the TV
more closely so first we treat the TV tap the TV samples with antibiotics and
tap the TV samples with antibiotics and
tap the TV samples with antibiotics and then somehow create a sensor to measure
then somehow create a sensor to measure
then somehow create a sensor to measure the change in protein secretion in the
the change in protein secretion in the
the change in protein secretion in the sensor for example t detects an increase
sensor for example t detects an increase
sensor for example t detects an increase in the amount of protein that is
in the amount of protein that is
in the amount of protein that is secreted that is consider is that the TV
secreted that is consider is that the TV
secreted that is consider is that the TV samples is drug-resistant now on the
samples is drug-resistant now on the
samples is drug-resistant now on the other hand in the TV samples
other hand in the TV samples
other hand in the TV samples does not detect an increase in the
does not detect an increase in the
does not detect an increase in the amount of proteins and it is therefore
amount of proteins and it is therefore
amount of proteins and it is therefore the bacterias therefore is no longer
the bacterias therefore is no longer
the bacterias therefore is no longer metabolically active and therefore
metabolically active and therefore
metabolically active and therefore is susceptible to the drug and hence not
is susceptible to the drug and hence not
is susceptible to the drug and hence not drug resistant although the actual
drug resistant although the actual
drug resistant although the actual research has much more complexities and
research has much more complexities and
research has much more complexities and terminologies involved the core idea to
terminologies involved the core idea to
terminologies involved the core idea to be sure is that we are trying to use an
be sure is that we are trying to use an
be sure is that we are trying to use an indirect method to determine the level
indirect method to determine the level
indirect method to determine the level of activeness of the bacteria and using
of activeness of the bacteria and using
of activeness of the bacteria and using that measurement to indicate with effect
that measurement to indicate with effect
that measurement to indicate with effect the bacteria is drug resistant
the bacteria is drug resistant
the bacteria is drug resistant eliminating the requirement for
eliminating the requirement for
eliminating the requirement for observable growth which is too
observable growth which is too
observable growth which is too time-consuming but this idea in mind I
time-consuming but this idea in mind I
time-consuming but this idea in mind I started to devise my own DIY solutions
started to devise my own DIY solutions
started to devise my own DIY solutions to transform normal TV samples into a
to transform normal TV samples into a
to transform normal TV samples into a single cell suspension using medical
single cell suspension using medical
single cell suspension using medical needles and simple white sand filters
needles and simple white sand filters
needles and simple white sand filters and then the next step was dead
and then the next step was dead
and then the next step was dead determining what type of TV proteins to
determining what type of TV proteins to
determining what type of TV proteins to detect and so after weeks of scouring
detect and so after weeks of scouring
detect and so after weeks of scouring through lists of hundreds and hundreds
through lists of hundreds and hundreds
through lists of hundreds and hundreds of TV proteins I finally saw the protein
of TV proteins I finally saw the protein
of TV proteins I finally saw the protein that was looking for called MPT 6 before
that was looking for called MPT 6 before
that was looking for called MPT 6 before the unique property of this protein is
the unique property of this protein is
the unique property of this protein is that it is secreted only by tuberculosis
that it is secreted only by tuberculosis
that it is secreted only by tuberculosis though especially in its active State
though especially in its active State
though especially in its active State now I’ve constructed my own bio sensor
now I’ve constructed my own bio sensor
now I’ve constructed my own bio sensor utilizing electrochemical reactions to
utilizing electrochemical reactions to
utilizing electrochemical reactions to detect the change in the protein
detect the change in the protein
detect the change in the protein secretion so in the detection process
secretion so in the detection process
secretion so in the detection process e-every t-64 protein is transducers into
e-every t-64 protein is transducers into
e-every t-64 protein is transducers into an electrical current and so the higher
an electrical current and so the higher
an electrical current and so the higher the electrical current measurement he
the electrical current measurement he
the electrical current measurement he hired the amount
hired the amount
hired the amount and before 30 and honestly mama doing
and before 30 and honestly mama doing
and before 30 and honestly mama doing all this research there were numerous
all this research there were numerous
all this research there were numerous obstacles along the way initially I said
obstacles along the way initially I said
obstacles along the way initially I said a research proposal to various research
a research proposal to various research
a research proposal to various research institutions in Thailand we see me back
institutions in Thailand we see me back
institutions in Thailand we see me back countless refusals because everyone deed
countless refusals because everyone deed
countless refusals because everyone deed that was impossible for a high school
that was impossible for a high school
that was impossible for a high school student like myself to tackle with such
student like myself to tackle with such
student like myself to tackle with such dangerous enemies of research in
dangerous enemies of research in
dangerous enemies of research in addition while I was doing my research I
addition while I was doing my research I
addition while I was doing my research I had to skipped over 150 classes often
had to skipped over 150 classes often
had to skipped over 150 classes often working at the laboratory from 8:00 in
working at the laboratory from 8:00 in
working at the laboratory from 8:00 in the morning to late at night because of
the morning to late at night because of
the morning to late at night because of amatory
amatory
amatory was over 100 kilometers away from
was over 100 kilometers away from
was over 100 kilometers away from Bangkok and like other researchers out
Bangkok and like other researchers out
Bangkok and like other researchers out there and I encountered numerous
there and I encountered numerous
there and I encountered numerous obstacles and failures however what the
obstacles and failures however what the
obstacles and failures however what the exacerbated off of me was the fact that
exacerbated off of me was the fact that
exacerbated off of me was the fact that I had literally placed everything on the
I had literally placed everything on the
I had literally placed everything on the line my grades my time and all the
line my grades my time and all the
line my grades my time and all the effort that could possibly gather and so
effort that could possibly gather and so
effort that could possibly gather and so the question that kept haunting me
the question that kept haunting me
the question that kept haunting me throughout this process is whether I
throughout this process is whether I
throughout this process is whether I have basically you risk everything
have basically you risk everything
have basically you risk everything whether I have sacrificed everything for
whether I have sacrificed everything for
whether I have sacrificed everything for essentially nothing those times were
essentially nothing those times were
essentially nothing those times were definitely one of the most difficult
definitely one of the most difficult
definitely one of the most difficult times of my high school commute and so
times of my high school commute and so
times of my high school commute and so after overcoming those obstacles and
after overcoming those obstacles and
after overcoming those obstacles and spending over a year inventing and
spending over a year inventing and
spending over a year inventing and reinventing when you guys I finally saw
reinventing when you guys I finally saw
reinventing when you guys I finally saw the light at the end of the tunnel as I
the light at the end of the tunnel as I
the light at the end of the tunnel as I discovered that my sensor can detect
discovered that my sensor can detect
discovered that my sensor can detect drug-resistant TB within two to three
drug-resistant TB within two to three
drug-resistant TB within two to three days which is ten times faster than
days which is ten times faster than
days which is ten times faster than expected and to ensure whether my sensor
expected and to ensure whether my sensor
expected and to ensure whether my sensor would reach the people who need it most
would reach the people who need it most
would reach the people who need it most I further design it such that it is
I further design it such that it is
I further design it such that it is portable and also a low cost acid it’s
portable and also a low cost acid it’s
portable and also a low cost acid it’s based on abundant materials like carbon
based on abundant materials like carbon
based on abundant materials like carbon and although in research has won many
and although in research has won many
and although in research has won many national and international
national and international
national and international two words the real value of those
two words the real value of those
two words the real value of those recognition is that implementation was
recognition is that implementation was
recognition is that implementation was after cross validating my device I have
after cross validating my device I have
after cross validating my device I have plants work with me waho to possibly
plants work with me waho to possibly
plants work with me waho to possibly implement my research in the rural areas
implement my research in the rural areas
implement my research in the rural areas of Thailand and possibly in the future
of Thailand and possibly in the future
of Thailand and possibly in the future in other TV endemic regions of the world
in other TV endemic regions of the world
in other TV endemic regions of the world as well now my ultimate goal is that
as well now my ultimate goal is that
as well now my ultimate goal is that what they no longer will do paralysis
what they no longer will do paralysis
what they no longer will do paralysis death toll be comparable to those of
death toll be comparable to those of
death toll be comparable to those of natural disasters because if you look at
natural disasters because if you look at
natural disasters because if you look at it tuberculosis is entirely preventable
it tuberculosis is entirely preventable
it tuberculosis is entirely preventable it is entirely curable so why shouldn’t
it is entirely curable so why shouldn’t
it is entirely curable so why shouldn’t be taking away countless lives like
be taking away countless lives like
be taking away countless lives like earthquakes tsunamis that all those
earthquakes tsunamis that all those
earthquakes tsunamis that all those devastating natural disasters and so
devastating natural disasters and so
devastating natural disasters and so looking back at all those experiences as
looking back at all those experiences as
looking back at all those experiences as I realize that you don’t actually have
I realize that you don’t actually have
I realize that you don’t actually have to be a college professor with multiple
to be a college professor with multiple
to be a college professor with multiple degrees to have new ideas valid to be
degrees to have new ideas valid to be
degrees to have new ideas valid to be honest with you when I started to do my
honest with you when I started to do my
honest with you when I started to do my TV research I had a ferry in India when
TV research I had a ferry in India when
TV research I had a ferry in India when tuberoses was accept two main things
tuberoses was accept two main things
tuberoses was accept two main things that TV kills people and that he has
that TV kills people and that he has
that TV kills people and that he has something to do with the human lungs and
something to do with the human lungs and
something to do with the human lungs and so athletes are you guys right now the
so athletes are you guys right now the
so athletes are you guys right now the audience might know more about
audience might know more about
audience might know more about tuberculosis than I did that thing and
tuberculosis than I did that thing and
tuberculosis than I did that thing and so the key takeaway is that it is not
so the key takeaway is that it is not
so the key takeaway is that it is not about how much you know but how willing
about how much you know but how willing
about how much you know but how willing you are to explore beyond you are
you are to explore beyond you are
you are to explore beyond you are though because those areas are where the
though because those areas are where the
though because those areas are where the magic happens
magic happens
magic happens and so today today today I’m not trying
and so today today today I’m not trying
and so today today today I’m not trying to sway you guys become geeky scientists
to sway you guys become geeky scientists
to sway you guys become geeky scientists or crazy inventions like myself because
or crazy inventions like myself because
or crazy inventions like myself because each and every one of you have your own
each and every one of you have your own
each and every one of you have your own unique passions all I’m asking of you is
unique passions all I’m asking of you is
unique passions all I’m asking of you is to ask what hard work and determination
to ask what hard work and determination
to ask what hard work and determination to your existing passion and hopefully
to your existing passion and hopefully
to your existing passion and hopefully found your passion will not require you
found your passion will not require you
found your passion will not require you to skip over 150 classes like I said
to skip over 150 classes like I said
to skip over 150 classes like I said thank you
thank you
thank you [Applause]
”
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