launch a new product on the market
you need to know if it is safe for the
consumer
if it will not irritate the skin or cause
allergic contact dermatitis is an
quite frequent reaction caused by
sensitizing substances that are
those who come into contact with the
skin
Let’s trigger an answer.
that will cause blisters
spots and skin pain in a company
cosmetics wants their products
cause this certain symptom
then you need to pre-investigate
your products are safe but like
know the cosmetic ingredient where
or do not cause contact dermatitis
the beginning of the last century
researchers developed the
experimental skin sensitization
which are tests using animals
mainly by quinho of India for
try to predict whether the substance is
do not cause contact dermatitis
allergic
on the assumption that if a
substance causes allergy and in animals
likely to cause
human beings
in these tests the animals’ hairs are
removed and the substance is tested
applied to the skin
after a few days the substance is
applied and observing if reddening
swelling on the spot
if any we understand that it is a
sensitizer or is likely to
that product will cause a reaction
allergy in the skin of humans
beyond the guinea pigs and say if
passages are not pigs or
originating in India
many other animals like rats rabbits
and mice were and still are today
widely used as models of
research scientific models but the whole
model is a representation of a
simplified idealization and not a
exact copy
the use of animals as models
I’ve been getting more and more criticized
not only by animal advocates
but also by many scientists as the
my scientific mentor the teacher
thales stress that the
animal experimentation should be
understood as an obstacle to progress
scientifically , one can imagine that
immune system of a mouse from a
rabbit is not exactly the same as
human being
although they have many similarities but
are just the differences that can
mislead the tests and
the most dangerous are called false
negative when a substance does not
causes an allergic reaction and an animal
but the human being when applying a
with that same substance
on the skin will develop a reaction
allergic reaction that may even be
quite serious
Good testing with India shares
were recognized in the 1980’s and
have been used in
assessment that the safety of
cosmetics by several companies
but many countries like India, Israel
and many all members of the union
Europe have already banned not only the use of
animals in tests but also the
marketing of tested products
in animals, which may be a barrier
for Brazilian products
Unfortunately I’m still in Brazil.
allows the performance of these tests
but can you eliminate the
use of animals
opposition to the use of animals in
medical research already had its
representatives there in 1801 bit
but the question of the use of animals to
cosmetic tests or the strength and
notoriety in England in the
80 when activists made public
the cruelty involved in the
cosmetic industry again says rita
excuse is good from there we
we have social pressure for the end of
use of animals in the tests and
Worldwide researchers began to
dedicate itself to the development of
alternative methods
alternative methods often
are understood as the methods
who do not use animals but the term
refers to a concept coined by
researchers of your boat at the end of
that to say that it is the
concept the principle of the three Rs one
reduction is reduced.
number of animals in the tests the other r
the refinement of techniques with the
goal of causing less stress and
suffering in these animals and the third r
of English replays that would be the
replacement of the use of live models
by other methods
the first alternative method to the use of
animals to be validated to be validated
was the local lymph node assay
he is known by lln to this method
uses mice is a method
alternative because applying two of the
three erres the reduction of the number of
and refinement of the technique by
which causes less suffering in
comparison with the previous method
good but whenever I said that the template
it ‘s not a perfect representation
who has ever had allergy to jewelry
It is quite frequent right?
this energy is usually caused by
nickel then nickel is a
allergen for the human being but he
used to be a false negative in these
animal testing
nowadays we know why this
we do know some
differences between the immune system of
mice and the human being but the
differences that we have not yet
We know
for sensitizing substances
were no longer identified in the tests
with animals to be placed in the
market causing allergies in humans
is there no other way
to do these tests something more
reliable but the most similar model
with the human being than a mouse
but how to know if a substance can
or not cause allergies in organisms
because the allergic process is
something so complex
The first is to understand what
happens on the skin
when in contact with the substance
chemistry we’ve been testing since
penetration of this ingredient
through the layers of the skin
combination of this substance with the
different proteins the tissue
activation of different types
cells such as keratinocytes and
dendritic cells until the activation
of the immune system
this is a substance
sensitizing
memory cells that will act in a
next contact with that same
substance generating the symptoms that I
I commented understanding these steps is
possible to appropriate the advances
to simulate the laboratory
what happens on the skin
when you contact these
substances linking knowledge and
technology to develop methods in
animals in avon cosmetic tests
order that third of the r
replacement
the cosmetic tests begin to make use of
the glass methods which are the
cell cultures in the laboratory
including human cells and in the year
past was recognized the first
alternative in vitro method
animal testing for
skin sensitization and other
methods have been developed and
should be linked to other methods in
glass and also chemical and
to compose battery packs
integrated test batteries capable of
provide even more accurate results
reliability of the tests performed with
animals
It is good to think then that if we
we are making these batteries
substitute methods using cultures
of human cells
We are testing cosmetics without
cause certain animal suffering
good was what I thought until entry
doctorate and realize starting
search more
I realized that many methods in glass
alternatives to animal testing
use different products of origin
animal of different species
the one between them that caused me
indignation from my first day in the
laboratory during the training
master’s degree in biotechnology in
University of Sao Paulo
this product routinely used in
cell culture laboratories
is the fetal bovine serum, that’s what
Are you imagining whey she leaves?
Liquid fetal bovine blood rather
ox and baby cows parties
well you’ve never seen a cow fetus
donating blood right
So how will the serum come out?
the fetus and arrives at the laboratory
government has done this is obtained from the blood of
fetuses removed from their mothers’ womb
during slaughter for human consumption
blood collection is done by function
cardiac
using a needle is inserted between
the ribs of the fetus straight into the heart
which should continue beating
this procedure is done without anesthesia
and the animal bleeds to death
this procedure leads to death around
1 to 2 million animals every year
to supply the serum demand of the
cell culture laboratories of the
All the world
serum is used as a supplement to the
culture medium to provide various
components such as factors
growth are essential for the
cells grow if the
laboratory flasks and
It is worth noting that in addition to
ethics involved in the use of
there are also some problems
scientific technicians
change of tests on live animals
for more modern methods it was not
something spontaneous did not happen overnight
for the day
in the case of awareness tests
we start with the
guinea pigs understand exactly
the process just by looking at the
or not of the symptoms
we went on to the tests with mice
an alternative method using those
two erres with less animals and techniques
the more refined we come in the
current tests but still use some
components of animal origin shall not be
we invest so much and we research
both we have so much knowledge acquired
and high technology involved but
and yet it causes so much suffering to
animals
although we have greatly improved the
quality of these tests
will we have no way out of a
has as it has totally cosmetics
free from cruelty
It was a good indignation that led me to
propose a research project to
compete for the main prize
international cooperation in the area of
alternatives
I was in the middle of my doctorate when
my friend jaqueline torres insisted
to send a project to the
snack beaches and 2015 in the youth category
researcher
I decided that I would try to change what I already
It bothers me a lot of time and that
seemed even more absurd in the area of
alternative methods
I could not understand how it was
alternative methods to
use of animals using
animal origin whose production is so
cruel because no one was worried
with the use of fetal bovine serum
it seems that the researchers are so
concerned about the technical details
of their experiments that do not
concerns like those reagents that
they were obtained were obtained and
people fighting to end the use of
animals in the tests
to know that the methods use
human cells understand that they do not
there is good and
so the use of the federal government serum in
methods and glass was not being
questioned
but I’m an activist scientist one
activist scientist I do not know what to
more discontented was to know that
there are alternatives to using serum already
I had been researching this for some years
before and he knew that it was
possible to grow human cells in
other culture media already available
commercially and which are fully
free of products of animal origin
good if there are alternatives why not
try to make the methods in glass
totally free from cruelty
unfortunately the jurors of the snack price
and they agreed with me and gave me the
prize that funded the research that
is being performed in inmetro that
state of rio de janeiro and one of the
central laboratories
the national network of alternative methods
[Music]
the goal of the research is to eliminate the use
of the federal government serum and the in
validated by the tests of
skin sensitization
for this we are testing
different chemically defined media
and a process that consists of reducing
gradually the amount of serum and
select cells capable of
survive in this new environment
without any component of animal origin
the next step is whether these
adapted cells continue to have
ability to identify
sensitizers just as it happens with
the cells cultured in the medium with serum
of the original method
good right after the goal is to eliminate
other components of animal origin
this method and do the same with other
methods in glass so that we can have
safe and free cosmetics
animal suffering is totally free from
cruelty
we are in a moment of the passwords in
that it is necessary to review the
we’re doing no more
It’s worth asking if it’s worth it.
to use an animal in a cosmetic test
or does not matter what contribution
supposedly they can bring us right
what would be the best model for
test a cosmetic animal cells
human
is not the safest way to know the
that will happen in humans is tested
in humans and less than one hundred years we
we had scientists who performed
experiments and human beings in
science name
good today it is obvious to us that being
should only participate in a
scientific research if it does not
no harm and if he agrees to
participate but the animals
did they offer themselves as
volunteers because we give ourselves the
right to use animals for research
the scientific community has already recognized
in animal science at least 12
remembered if the ability to have
experiences of reacting a stimulus of
conscious form feel happiness odor
anyone here doubts that a dog is capable
to consciously feel pain and a
cow or a mouse
we tend to think that the other is the
another only feels if he reacts as we
but we humans so evolved species
or are we able to hear the cries of
pain emitted by some rodents but
This does not mean that they do not
we do not agree with
experiments that could harm
human beings independent of
justifications
why then will it still be
we harm animals on behalf of the
science
unfortunately it ‘s soup there are
Scientists who favor the
animal experimentation they claim that
they care about the well-being of these
animals
but if we agree that they have the
right not to suffer because they do not
we are respecting their right not to
participate in the survey because they
we respect their right to own
life
unfortunately there are scientists
the end of the use of animals in the
science
I need to say that working all
days for the change I want
see in the world is an honor a joy and
a privilege but will we
unity we are doing our part
if we agree that causing suffering
unnecessary in animals
something that should not happen because the
most of us do not put into practice
day by day
the animals are exploited and tortured
for the most different purposes
beyond science and sports and
entertainment as in circuses and
zoos in the production of clothing
leather and wool
transport and food
In fact, most of us still
feeds animals even knowing that the
animals for consumption
human is something so cruel
it is not only a question of respecting the
Until the UN recommends eliminating
products of animal origin
lamentation is proven
beneficial to our health and to the
planet as a whole
we are in a moment of transition
important event our relationship with
the environment and animals
rethinking concepts changing habits
it is clear that major changes
happen overnight, but the
animals do not exist for our
benefit
they have the right not to be
tortured and killed, and we
we have the duty and ethical obligation to
not cause suffering to them not only
scientists but all of us and our
daily attitudes are part of the
change
thanks
[Applause]
”
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